LOCUS       ISCR3-tetA(G)-floR_unit 6183 bp    DNA     linear       22-OCT-2020
DEFINITION  Almonella enterica.
ACCESSION
VERSION
KEYWORDS    .
SOURCE      almonella enterica
  ORGANISM  almonella enterica
            Unclassified.
REFERENCE   1  (bases 1 to 6183)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of ISCR3-tetA(G)-floR unit
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6183)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-OCT-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: newbler v. 2.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6183
                     /organism="almonella enterica"
                     /mol_type="genomic DNA"
     mobile_element  1..6183
                     /gene="ISCR3-tetA(G)-floR unit"
                     /product="Putative resistance unit: ISCR3-tetA(G)-floR
                     unit"
     mobile_element  complement(1..1981)
                     /gene="ISCR3"
                     /product="Insertion sequence: truncated ISCR3"
     CDS             205..1737
                     /gene="tnpA"
                     /codon_start=1
                     /transl_table=11
                     /product="ISCR3 tansposase"
                     /translation="MPRLATSRRQAAGCAPLPSAHTGSRYARHAPERTLLYALVEAHY
                     PDFIARIEAEGRSLPGYVREAFDAYLRCGVLEHGFLRVVCEHCRAERLVAFSCKKRGF
                     CPSCGARRMAESARHLVEEVFGPRPVRQWVLSFPYPLRFLFASKPEAIGPVLGIVQRV
                     IAGWLADQAGIDRASAQCGAVTLIQRFGSALNLNIHFHMLWLDGVYVEATELPRRELR
                     LHRARAPTTAQLTQLAATIAHRVCRHLTRKGWLEGEGESAFLADSAAGDDSMDGLRMS
                     SITYRIATGRDAGCKVVTLQTLPGDAGSLEGEAGKVGGFSLHAGVAAEAHESHKLEKL
                     CRYITRPAISEKRLSIALQGRVRYQLKTPWRNGTTHVEWDPVDFIAKLAALVPPPRAH
                     LTRFHGVFAPNANLRAQLTPSGRGKRPAGDAAPVDVSAHDAPRSPEEKRRAMSWAQRL
                     KRVFSIDVTACVHCGGTVRIVASIEEPTAIRAILAHFEKHGAREEAHYRPAARAPPVQ
                     AA"
     CDS             complement(1829..2620)
                     /gene="lysR"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /translation="MRRLRRNDGLNLLSNHLLSGRVPMMTLVHTLAVAEYLNFRHAAN
                     ALGVAQSSVSARVKALEEDLGILLFERHARGVRLTEAGRHFVERIAVGIDQLDHAVKT
                     AGMAAAGESGRLRIGIHALIPHSFLAKLIGQYRKDYPDVEVEIAEGPAREAVVQLRAG
                     RLDVAFVAGTPQPPDCHSRRTWTEPLLAVLPERHPLAKRSAVTWPDLAGETFLAYRKF
                     KRPFRAVCWVGGGRASPVLGTDRTSVSGISASRGHAAALSAAMLR"
     misc_recomb     1961..1981
                     /gene="oriIS"
                     /product="oriIS of ISCR3"
     CDS             complement(2641..3816)
                     /gene="tetA(G)"
                     /codon_start=1
                     /transl_table=11
                     /product="Tetracycline efflux protein TetA, class G"
                     /translation="MRSSAIIALLIVGLDAMGLGLIMPVLPTLLRELVPAEQVAGHYG
                     ALLSLYALMQVVFAPMLGQLSDSYGRRPVLLASLAGAAVDYTIMASAPVLWVLYIGRL
                     VSGVTGATGAVAASTIADSTGEGSRARWFGYMGACYGAGMIAGPALGGMLGGISAHAP
                     FIAAALLNGFAFLLACIFLKETHHSHGGTGKPVRIKPFVLLRLDDALRGLGALFAVFF
                     IIQLIGQVPAALWVIYGEDRFQWNTATVGLSLAAFGATHAIFQAFVTGPLSSRLGERR
                     TLLFGMAADATGFVLLAFATQGWMVFPILLLLAAGGVGMPALQAMLSNNVSSNKQGAL
                     QGTLTSLTNLSSIAGPLGFTALYSATAGAWNGWVWIVGAILYLICLPILRRPFATSL"
     CDS             3920..4546
                     /gene="tetR(G)"
                     /codon_start=1
                     /transl_table=11
                     /product="Tetracycline repressor protein TetR, class G"
                     /translation="MTKLDKGTVIAAALELLNEVGMDSLTTRKLAERLKVQQPALYWH
                     FQNKRALLDALAEAMLAERHTRSLPEENEDWRVFLKENALSFRTALLSYRDGARIHAG
                     TRPTEPNFGTAETQIRFLCAEGFCPKRAVWALRAVSHYVVGSVLEQQASDADERVPDR
                     PDVSEQAPSSFLHDLFHELETDGMDAAFNFGLDSLIAGFERLRSSTTD"
     CDS             complement(4753..5967)
                     /gene="floR"
                     /codon_start=1
                     /transl_table=11
                     /product="Chloramphenicol/florfenicol resistance, MFS
                     efflux pump"
                     /translation="MTTTRPAWAYTLPAALLLMAPFDILASLAMDIYLPVVPAMPGIL
                     NTTPAMIQLTLSLYMVMLGVGQVIFGPLSDRIGRRPILLAGATAFVIASLGAAWSSTA
                     PAFVAFRLLQAVGASAMLVATFATVRDVYANRPEGVVIYGLFSSMLAFVPALGPIAGA
                     LIGEFLGWQAIFITLAILAMLALLNAGFRWHETRPLDQVKTRRSVLPIFASPAFWVYT
                     VGFSAGMGTFFVFFSTAPRVLIGQAEYSEIGFSFAFATVALVMIVTTRFAKSFVARWG
                     IAGCVARGMALLVCGAVLLGIGELYGSPSFLTFILPMWVVAVGIVFTVSVTANGALAE
                     FDDIAGSAVAFYFCIQSLIVSIVGTLAVTLLNGDTAWPVICYATAMAVLVSLGLALLR
                     SRDAATEKSPVV"
BASE COUNT     1351 a   1915 c   1884 g   1033 t
ORIGIN
        1 gaaccggacc gcgccagcgc ggtccgatcc cggcaacgac ccgacatcaa ggccccaagg
       61 acggggccgg agcccggcag cgatgccggg ctttttgttg tgcccgcgcc gcggcaatgt
      121 ctgacgcgaa gatcagaacg caccgatacg aacgtgcgaa cacaggcgca acactgagca
      181 gccgtccccg caccggagcg ctgcgtgccg cgcctcgcca catcccggcg gcaagccgcg
      241 ggatgcgcgc cactgccgtc cgcccacacc ggttcgcggt acgcgcgcca cgcgcccgag
      301 cgcacgctgc tgtacgcgtt ggtagaggcg cactacccgg acttcattgc acggatcgaa
      361 gcggagggcc gctcgctgcc cgggtatgtc cgcgaggcgt tcgatgccta cctgcgttgc
      421 ggcgtactcg agcacggctt cctgcgggtg gtgtgcgagc actgccgtgc agagaggctg
      481 gtggccttct cctgcaagaa gcgcgggttc tgcccgagtt gcggcgcgcg acgcatggcc
      541 gagagtgcgc ggcacctggt cgaggaggtg ttcggcccgc ggcctgtgcg gcaatgggtg
      601 ctgagctttc cgtacccctt gcgtttcctg ttcgccagca agccagaagc cattggcccg
      661 gtgctgggca tcgtgcagcg cgtgatcgcc ggctggttgg ccgatcaagc cggcatcgac
      721 cgcgccagcg cccagtgcgg cgcggtgacg ctgatccagc gtttcggcag cgcgctgaac
      781 ctgaacatcc acttccacat gctgtggctc gacggcgtgt acgtggaagc caccgagctg
      841 ccgcggcgcg aactgcgcct gcaccgcgcc cgtgcgccca ccaccgcgca gttgacccag
      901 ctggcagcta ccatcgcgca ccgggtgtgt cggcacctga cgcgcaaagg ctggctcgaa
      961 ggggagggcg aatcggcctt cctggcagac agcgctgcag gcgacgacag catggatggg
     1021 ctgcggatga gttcgatcac ctaccgcatc gccaccggcc gcgacgctgg ctgcaaggtc
     1081 gtcacgctgc aaacgctgcc cggtgacgcc ggttcgctgg agggcgaagc cggcaaggtc
     1141 ggcggcttct cactgcatgc cggcgtggcg gccgaagcac acgaaagcca caagctggaa
     1201 aagctgtgcc gctacatcac gcgcccggcg atcagcgaga agcggctgtc gatagcgctc
     1261 cagggcaggg tgcgttacca gctcaagacc ccgtggcgca atggcaccac gcatgtggaa
     1321 tgggatccgg tggatttcat cgccaagctg gcggcgctgg tcccgccacc tcgcgcgcat
     1381 ctcacccgct tccacggcgt attcgccccg aatgcaaacc tgcgtgcgca gctgacgccc
     1441 tcggggcgcg gcaagcggcc tgcgggcgat gcggcgccag tggacgtcag cgcccacgac
     1501 gcgccgcgca gccccgagga gaagcgccgt gcgatgagct gggcgcaacg gctcaagcgg
     1561 gtcttttcca tcgacgtcac cgcctgcgtc cactgcggtg gcaccgtgcg gatcgtcgcc
     1621 agcatcgagg aacccaccgc catccgcgcc atcctcgccc acttcgagaa gcacggcgcg
     1681 cgggaagaag cgcactacag gcccgcagcg cgcgcgccgc cagtgcaagc cgcgtgacga
     1741 tctgccggct gcacagccga cggcgaaacc ggaatccgag ccgatgcggc cacgatccgc
     1801 agggcggcgc tcggcccgct gtcgggaatc agcgaagcat ggctgctgac aacgccgctg
     1861 cgtggccccg cgatgccgaa atcccactca cagacgtccg atccgtgccc aaaacggggc
     1921 ttgcgcgacc gccgcctacc cagcagactg cccgaaaagg gcgtttgaac ttcctatacg
     1981 caaggaacgt ctcgcctgcc aaatcgggcc atgtgacggc tgaccgcttg gcgagcggat
     2041 gccgttccgg tagcaccgcc aagagcggtt cggtccatgt gcgacgggaa tggcagtcgg
     2101 gtggttgggg cgtgcccgcg acgaacgcca cgtccaacct gccggcgcga agctgcacca
     2161 ccgcttcacg ggccgggcct tcggcgatct cgacttcaac atcggggtaa tccttgcggt
     2221 attggccgat cagctttgcg aggaagctat gcggaatcag ggcatggata ccgatacgaa
     2281 gccggccgct ttctccggct gccgccatgc cggcggtttt caccgcatgg tcgagttggt
     2341 caatacctac ggctatccgc tcgacgaaat ggcgtccggc ctcggtcagc cgaacgccgc
     2401 gcgcatgacg ctcgaacaag aggatgccga ggtcttcttc cagtgccttc acgcgggcgc
     2461 tgacgctgga ctgtgcaacg ccgagcgcgt tggcggcgtg acggaagttg agatattcgg
     2521 cgacagcgag agtgtgaact aaggtcatca ttggcactcg ccccgaaagg agatgattgc
     2581 tcaacagatt taatccgtca tttctcctaa gtctacgcat gccaaatcgc catgactaaa
     2641 tcacaatgaa gttgcgaatg gtctgcgtag tattggcaga catattaaat agaggatcgc
     2701 gccgacaatc caaacccaac cgttccatgc cccggcggtg gcagaataga gtgctgtgaa
     2761 gccaagcggt cctgcgatag agcttagatt ggtgaggctc gttagcgttc cttgcaaagc
     2821 cccttgcttg ttactgctga cattgtttga gagcattgcc tgcaaggccg gcatgccaac
     2881 acccccggcg gcaagcagca acagaatcgg gaacaccatc catccctgcg tggcaaaagc
     2941 cagaagaacg aagccagtcg catccgcagc catgccaaac agcagcgtgc gccgctctcc
     3001 aagccggctt gaaagcgggc cggtaacaaa cgcttggaag atcgcatgtg ttgccccaaa
     3061 cgccgcgagc gacaaaccaa cggtcgcggt gttccactga aaacggtcct cgccatatat
     3121 gacccatagg gctgcaggca cttggccgat cagttgaata atgaagaaaa ctgcgaaaag
     3181 cgcacctagc ccgcgcaatg catcatccag ccgtaacaga acgaatggtt tgatgcgaac
     3241 cggctttccg gtcccgccat ggctgtgatg agtctccttg aggaaaatgc aggcaagcag
     3301 gaacgcgaac ccgttgagaa gggcggcggc gataaacggg gcatgagcag agataccacc
     3361 gagcatgcca ccaagtgctg gcccggcaat catgcccgcc ccataacagg cccccatgta
     3421 gccgaaccag cgtgcgcgag aaccttcccc cgtcgaatcg gcaatggttg aggctgctac
     3481 agctccggtt gcgcccgtga cgccggacac gagtcggccg atatagagca cccataagac
     3541 cggcgctgat gccataatcg tgtaatcgac tgcggctcct gcaagagaag ccagaagtac
     3601 cggacgccga ccgtaagaat ccgaaagctg tccaagcatg ggcgcgaaga cgacctgcat
     3661 caatgcatag agcgacagca aggcaccata gtgtccagcg acctgctctg ctggcacaag
     3721 ctcacgcaga agcgtcggaa ggacgggcat gatgaggccg agacccatgg cgtcaagacc
     3781 cacgatcagc agggcaatga tggcagagct gcgcacctga aactccagcg ccgctcaatg
     3841 gagcgacttt atcaacgata aggagatgga catataactt atcggtgata aattgtcaag
     3901 cactggcgaa ggaacgtgaa tgaccaaact ggacaagggc accgtgatcg cggcggcgct
     3961 agagctgttg aacgaggttg gcatggacag cctgacgacg cggaagctcg ctgaacgcct
     4021 caaggttcag cagcctgcgc tttactggca tttccagaac aagcgagcgc tgcttgatgc
     4081 gctcgccgag gcgatgctgg cggaacgcca tacccgctcg ctacccgaag agaatgagga
     4141 ctggcgggtg ttcctgaaag agaatgccct gagcttcaga acggcgttgc tctcttatcg
     4201 ggacggcgcg cgtatccatg ccggcactcg accgacagaa ccgaattttg gcaccgccga
     4261 gacgcaaata cgctttctct gcgcggaggg cttttgtccg aagcgcgccg tttgggcgct
     4321 ccgggcggtc agtcactatg tggtcggttc cgttctcgag cagcaggcat ctgatgccga
     4381 tgagagagtt ccggacaggc cagatgtgtc cgagcaagca ccgtcgtcct tcctgcacga
     4441 tctgtttcac gagttggaaa cagacggcat ggatgctgcg ttcaacttcg gactcgacag
     4501 cctcatcgct ggtttcgagc ggctgcgttc atctacaaca gattagaggc ttatgcccct
     4561 ttgccgcccc aactgccacg acaccgatcc gctttgcacg atgcccatga cctcacggcc
     4621 gagctggcgg tcgatgaccg gccgccacgg gacaagggaa atgagcggta tcttgccaga
     4681 caggataccg ccattcacga ggtttcgaag attattgcgc cgcatcggag cgggcttgct
     4741 tccagtcgtc ggctagacga ctggcgactt ctcggtggca gcatcacggg atcgaaggag
     4801 cgccagcccc aacgacacca gcactgccat tgccgtggcg taacaaatca cgggccacgc
     4861 tgtatcgccg tttaacagcg tcaccgccaa tgtcccgacg atactgacta tcaggctttg
     4921 gatgcagaag tagaacgcga ccgctgatcc cgcgatgtcg tcgaactctg ccaaagcgcc
     4981 gttcgcggta acggacaccg tgaagacaat accgaccgcg acaacccaca tcggtaggat
     5041 gaaggtgagg aatgacggcg agccgtaaag ttcgccgatc cccaacagga ccgctccgca
     5101 aacaagcaac gccatcccac gcgccacgca tcctgcgatg ccccatctgg cgacaaagga
     5161 cttcgcgaaa cgggttgtca cgatcattac aagcgcgaca gtggcgaagg caaagctgaa
     5221 tccgatctcg gaatattccg cttggcctat gagcacacgg ggagccgtcg agaagaagac
     5281 gaagaaggtg cccataccgg cgctaaagcc gacagtgtaa acccaaaaag ccggactcgc
     5341 gaagatcggc aagacagatc ggcgcgtctt gacttgatcc agagggcggg tttcgtgcca
     5401 cctgaaaccc gcatttagga gtgcgagcat cgccagtata gccaaagtaa tgaatatcgc
     5461 ctgccatccc aagaactcgc cgatcaatgc tccggcgata gggccgagcg caggcacgaa
     5521 cgccagcatc gaactgaaaa ggccgtagat gacgacaccc tcaggacggt tggcataaac
     5581 gtcgcgaacc gtcgcgaacg tcgccaccag catggccgac gcgcccactg cttgaagtag
     5641 acggaaagcg acaaaggccg gtgcagttga agaccaagct gctcccagag acgcaatgac
     5701 gaaagccgtt gcgcccgcaa gtagaattgg ccgtcgcccg attctgtctg agagcggacc
     5761 aaaaatcacc tggcccacgc cgagcatcac catatagagg ctcaacgtga gttggatcat
     5821 agcgggcgtc gtgttcagga tgccgggcat cgctggaacg acagggagat aaatatccat
     5881 cgccagtgaa gcgaggatgt cgaaaggagc catcagcagc agtgctgccg gcagcgtata
     5941 ggcccacgcg gggcgtgtgg tggtcatgac gaatcaaccc tcgattaagg aataccgggc
     6001 gacgtctgct cgtcagcaat cagatgagac tagccttaca gagcgccgca acaacaatac
     6061 tggttgttgc ggcttacttg tctgctgact tggaatttcc catctgatta ctccacgctt
     6121 acgaatatga attgctacat tttatccgat tatcttttgc ttcgcaatgc ggcatgggcg
     6181 cac
//
